413 Effect of Hydrolysis and Polymerization on Bovine Beta-lactoglobulin Immunoreactivity
نویسندگان
چکیده
airway and is the most common chronic disease in children characterized by wheezing. Wheezing may occur even once it is up to 50% of all infants and children under 3 years old and topic dermatitis affecting over 10% of children. Objective: To define incidence and prevalence of asthma and others allergic diseases in population of less than 5 years of age in Juarez Hospital Mexico. Results: We evaluated 11,346 allergic patients on 4 years period (January 2007 to December 2010). Two thousand three hundred ninety six were #5 years (21.11%); 10.2% a year old or younger, 16.7% 2 years old, 18.5% had 3 years, 24.7% of 4 years and 30% 5 years old. Nine hundred ninety two patients (41.4%) were females and 1.404 (58.6%) male. One thousand two hundred thirty eight patients (51.7%) had only one diagnostic finding as most frequent cause of consultation allergic rhinitis (59.6%), followed by asthma (23%), atopic dermatitis (5.1%), prurigo by insect (4.3), immunodeficiency (1.2%) the rest had various pathologies such as contact dermatitis, gastro esophageal reflux, oral allergy syndrome, and others. The remaining patients 48.3% (1158) had more than one diagnostic. The most common associations were asthma and rhinitis 716 (30%), 72 patients (3%) with asthma and atopic dermatitis, 48 patients (2%) with some immune deficiency and asthma and/or rhinitis, 12 (0.5%) with prurigo and atopic dermatitis, 8 (0.3%) with urticaria and 11 patients (4.5%) with other diagnoses such as reflux or infections. Conclusions: Allergic diseases are common on pediatric population and children under 5 years old asthma and rhinitis is theprincipal cause of consultation.
منابع مشابه
In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization
OBJECTIVE To explore the use of β-lactoglobulin polymerized using microbial transglutaminase and heating to identify whether protein polymerization could reduce in vivo allergenicity and maintain in vitro and ex vivo immunoreactivity for use in tolerance-induction protocols. METHODS Based on previous protocols applied in mice and children, we performed in vivo challenges (using a skin prick t...
متن کاملEffect of genetic variation on the tryptic hydrolysis of bovine beta-lactoglobulin A, B, and C.
The structure, stability, and hydrolysis characteristics of beta-lactoglobulin (LG) A are different from those of either beta-LG B or beta-LG C. Purified samples of these proteins were hydrolyzed with trypsin and the rates of loss of native monomeric beta-LG structure were measured using sodium dodecyl sulfate PAGE. At the same time, the appearance of many individual peptides were identified an...
متن کاملEffect of glycation on the gastrointestinal digestibility and 1 immunoreactivity of bovine - lactoglobulin 2 3
متن کامل
Epitope mapping of a monoclonal antibody specific to bovine dry milk: involvement of residues 66-76 of strand D in thermal denatured beta-lactoglobulin.
beta-Lactoglobulin (beta-LG) is a bovine milk protein sensitive to thermal denaturation. Previously, we demonstrated that such structural change can be detected by a monoclonal antibody (mAb) specific to denatured beta-LG. In the present study, we show a dramatic increase in beta-LG immunoreactivity when heating raw milk between 70 and 80 degrees C. To map out the specific epitope of beta-LG re...
متن کاملInfluence of Surfactants on Lipase Fat Digestion in a Model Gastro-intestinal System
In the present study, we use a model gastro-intestinal system to study the influence of different food-grade surface-active molecules (Sn-2 monopalmitin, beta-lactoglobulin, or lysophosphatodylcholine) on lipase activity. The interfacial activity of lipase and surfactants are assessed with the pendant drop technique, a commonly used tensiometry instrument. A mathematical model is adopted which ...
متن کامل